Biomechanical Interactions of Stem Cells and Cancer Cells in the Tumor Microenvironment

My research combines molecular and gene expression analysis with quantitative biophysical analysis using sensitive mechanical tools (such as time-lapsed cell tracking, traction force microscopy, and particle tracking microrheology) to provide genetic and mechanical profiles of tumor and stromal cells in conditions that more closely mimic the tumor microenvironment. This approach has recently been used to demonstrate that ovarian cancer cells, which metastasize to the soft omentum fat pad, preferentially engraft on adipose-mimetic substrates or MSCs differentiated into soft adipocytes. Moreover, after engrafting they display a gene expression signature characteristic of epithelial-mesenchymal transition with corresponding increases in motility, proliferation, and chemoresistance.  Though this preference for soft matrices is in contrast to what has been documented in breast and other cancers, our studies have confirmed that an increased malignant phenotype is still associated with higher traction forces. Work from my lab has also shown that both murine and human MSCs undergo dramatic cytoskeletal stiffening in response to pro-migratory molecules in the tumor microenvironment, including a cocktail of molecules released by tumor cells in culture and individual molecules like TGF-1 and PDGF. The degree of stiffening is a key differentiating factor between MSCs and their less migratory fibroblast counterparts and even predictive of decreased MSC function with extended culture.

Mechanical properties of cells in the tumor microenvironment are dramatically altered by physical or chemical cues. These mechanical changes lead to differences in gene or protein expression and even cell function. Biophysical tools can be used to improve our understanding of how these factors contribute individually or collectively to the development of a malignant tumor microenvironment.